Two 14-3-3 proteins contribute to nitrogen sensing through the TOR and glutamine synthetase-dependent pathways in Fusarium graminearum.
Identifieur interne : 000016 ( Main/Exploration ); précédent : 000015; suivant : 000017Two 14-3-3 proteins contribute to nitrogen sensing through the TOR and glutamine synthetase-dependent pathways in Fusarium graminearum.
Auteurs : Elizabeth K. Brauer [Canada] ; Nimrat Manes [Canada] ; Christopher Bonner [Canada] ; Rajagopal Subramaniam [Canada]Source :
- Fungal genetics and biology : FG & B [ 1096-0937 ] ; 2020.
Abstract
Fusarium graminearum responds to environmental cues to modulate its growth and metabolism during wheat pathogenesis. Nitrogen limitation activates virulence-associated behaviours in F. graminearum including mycotoxin production and penetrative growth. In other filamentous fungi, nitrogen sensing is mediated by both the Target of Rapamycin (TOR) and the glutamine synthetase (GS)-dependent signaling pathways. While TOR-dependent nitrogen responses have been demonstrated in F. graminearum, the involvement of GS remains unclear. Our study indicates that both the TOR and GS signalling pathways are involved in nitrogen sensing in F. graminearum and contribute to glutamine-induced mycelial growth. However, neither pathway is required for glutamine-induced repression of the mycotoxin deoxynivalenol (DON) indicating that an additional nitrogen sensing pathway must exist. Further, two genes FgBMH1 and FgBMH2 encoding 14-3-3 proteins regulate nitrogen responses with effects on gene expression, DON production and mycelial growth. Unlike yeast, where 14-3-3s function redundantly in regulating nitrogen sensing, the 14-3-3 proteins have differing functions in F. graminearum. While both FgBMH1 and FgBMH2 regulate early glutamine-induced DON repression, only FgBMH2 is involved in regulating reproduction, virulence and glutamine-induced AreA repression. Together, our findings help to clarify the nitrogen sensing pathways in F. graminearum and highlight the involvement of 14-3-3s in the nitrogen response of filamentous fungi.
DOI: 10.1016/j.fgb.2019.103277
PubMed: 31605748
Affiliations:
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Brauer</name><affiliation wicri:level="1"><nlm:affiliation>Ottawa Research and Development Centre, Agriculture and Agri-Food Canada, Ottawa, ON K1A 0C6, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Ottawa Research and Development Centre, Agriculture and Agri-Food Canada, Ottawa, ON K1A 0C6</wicri:regionArea><wicri:noRegion>ON K1A 0C6</wicri:noRegion></affiliation></author><author><name sortKey="Manes, Nimrat" sort="Manes, Nimrat" uniqKey="Manes N" first="Nimrat" last="Manes">Nimrat Manes</name><affiliation wicri:level="1"><nlm:affiliation>Ottawa Research and Development Centre, Agriculture and Agri-Food Canada, Ottawa, ON K1A 0C6, Canada; Carleton University, Department of Biology, 1125 Colonel By Dr., Ottawa, ON K1S 5B6, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Ottawa Research and Development Centre, Agriculture and Agri-Food Canada, Ottawa, ON K1A 0C6, Canada; Carleton University, Department of Biology, 1125 Colonel By Dr., Ottawa, ON K1S 5B6</wicri:regionArea><wicri:noRegion>ON K1S 5B6</wicri:noRegion></affiliation></author><author><name sortKey="Bonner, Christopher" sort="Bonner, Christopher" uniqKey="Bonner C" first="Christopher" last="Bonner">Christopher Bonner</name><affiliation wicri:level="1"><nlm:affiliation>Ottawa Research and Development Centre, Agriculture and Agri-Food Canada, Ottawa, ON K1A 0C6, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Ottawa Research and Development Centre, Agriculture and Agri-Food Canada, Ottawa, ON K1A 0C6</wicri:regionArea><wicri:noRegion>ON K1A 0C6</wicri:noRegion></affiliation></author><author><name sortKey="Subramaniam, Rajagopal" sort="Subramaniam, Rajagopal" uniqKey="Subramaniam R" first="Rajagopal" last="Subramaniam">Rajagopal Subramaniam</name><affiliation wicri:level="1"><nlm:affiliation>Ottawa Research and Development Centre, Agriculture and Agri-Food Canada, Ottawa, ON K1A 0C6, Canada; Carleton University, Department of Biology, 1125 Colonel By Dr., Ottawa, ON K1S 5B6, Canada. Electronic address: rajagopal.subramaniam@canada.ca.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Ottawa Research and Development Centre, Agriculture and Agri-Food Canada, Ottawa, ON K1A 0C6, Canada; Carleton University, Department of Biology, 1125 Colonel By Dr., Ottawa, ON K1S 5B6</wicri:regionArea><wicri:noRegion>ON K1S 5B6</wicri:noRegion></affiliation></author></analytic><series><title level="j">Fungal genetics and biology : FG & B</title><idno type="eISSN">1096-0937</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Fusarium graminearum responds to environmental cues to modulate its growth and metabolism during wheat pathogenesis. Nitrogen limitation activates virulence-associated behaviours in F. graminearum including mycotoxin production and penetrative growth. In other filamentous fungi, nitrogen sensing is mediated by both the Target of Rapamycin (TOR) and the glutamine synthetase (GS)-dependent signaling pathways. While TOR-dependent nitrogen responses have been demonstrated in F. graminearum, the involvement of GS remains unclear. Our study indicates that both the TOR and GS signalling pathways are involved in nitrogen sensing in F. graminearum and contribute to glutamine-induced mycelial growth. However, neither pathway is required for glutamine-induced repression of the mycotoxin deoxynivalenol (DON) indicating that an additional nitrogen sensing pathway must exist. Further, two genes FgBMH1 and FgBMH2 encoding 14-3-3 proteins regulate nitrogen responses with effects on gene expression, DON production and mycelial growth. Unlike yeast, where 14-3-3s function redundantly in regulating nitrogen sensing, the 14-3-3 proteins have differing functions in F. graminearum. While both FgBMH1 and FgBMH2 regulate early glutamine-induced DON repression, only FgBMH2 is involved in regulating reproduction, virulence and glutamine-induced AreA repression. Together, our findings help to clarify the nitrogen sensing pathways in F. graminearum and highlight the involvement of 14-3-3s in the nitrogen response of filamentous fungi.</div></front></TEI><pubmed><MedlineCitation Status="In-Process" Owner="NLM"><PMID Version="1">31605748</PMID><DateRevised><Year>2020</Year><Month>07</Month><Day>31</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1096-0937</ISSN><JournalIssue CitedMedium="Internet"><Volume>134</Volume><PubDate><Year>2020</Year><Month>01</Month></PubDate></JournalIssue><Title>Fungal genetics and biology : FG & B</Title><ISOAbbreviation>Fungal Genet Biol</ISOAbbreviation></Journal><ArticleTitle>Two 14-3-3 proteins contribute to nitrogen sensing through the TOR and glutamine synthetase-dependent pathways in Fusarium graminearum.</ArticleTitle><Pagination><MedlinePgn>103277</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S1087-1845(19)30130-6</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.fgb.2019.103277</ELocationID><Abstract><AbstractText>Fusarium graminearum responds to environmental cues to modulate its growth and metabolism during wheat pathogenesis. Nitrogen limitation activates virulence-associated behaviours in F. graminearum including mycotoxin production and penetrative growth. In other filamentous fungi, nitrogen sensing is mediated by both the Target of Rapamycin (TOR) and the glutamine synthetase (GS)-dependent signaling pathways. While TOR-dependent nitrogen responses have been demonstrated in F. graminearum, the involvement of GS remains unclear. Our study indicates that both the TOR and GS signalling pathways are involved in nitrogen sensing in F. graminearum and contribute to glutamine-induced mycelial growth. However, neither pathway is required for glutamine-induced repression of the mycotoxin deoxynivalenol (DON) indicating that an additional nitrogen sensing pathway must exist. Further, two genes FgBMH1 and FgBMH2 encoding 14-3-3 proteins regulate nitrogen responses with effects on gene expression, DON production and mycelial growth. Unlike yeast, where 14-3-3s function redundantly in regulating nitrogen sensing, the 14-3-3 proteins have differing functions in F. graminearum. While both FgBMH1 and FgBMH2 regulate early glutamine-induced DON repression, only FgBMH2 is involved in regulating reproduction, virulence and glutamine-induced AreA repression. Together, our findings help to clarify the nitrogen sensing pathways in F. graminearum and highlight the involvement of 14-3-3s in the nitrogen response of filamentous fungi.</AbstractText><CopyrightInformation>Crown Copyright © 2019. Published by Elsevier Inc. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Brauer</LastName><ForeName>Elizabeth K</ForeName><Initials>EK</Initials><AffiliationInfo><Affiliation>Ottawa Research and Development Centre, Agriculture and Agri-Food Canada, Ottawa, ON K1A 0C6, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Manes</LastName><ForeName>Nimrat</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>Ottawa Research and Development Centre, Agriculture and Agri-Food Canada, Ottawa, ON K1A 0C6, Canada; Carleton University, Department of Biology, 1125 Colonel By Dr., Ottawa, ON K1S 5B6, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bonner</LastName><ForeName>Christopher</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Ottawa Research and Development Centre, Agriculture and Agri-Food Canada, Ottawa, ON K1A 0C6, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Subramaniam</LastName><ForeName>Rajagopal</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Ottawa Research and Development Centre, Agriculture and Agri-Food Canada, Ottawa, ON K1A 0C6, Canada; Carleton University, Department of Biology, 1125 Colonel By Dr., Ottawa, ON K1S 5B6, Canada. 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Brauer</name></noRegion><name sortKey="Bonner, Christopher" sort="Bonner, Christopher" uniqKey="Bonner C" first="Christopher" last="Bonner">Christopher Bonner</name><name sortKey="Manes, Nimrat" sort="Manes, Nimrat" uniqKey="Manes N" first="Nimrat" last="Manes">Nimrat Manes</name><name sortKey="Subramaniam, Rajagopal" sort="Subramaniam, Rajagopal" uniqKey="Subramaniam R" first="Rajagopal" last="Subramaniam">Rajagopal Subramaniam</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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